Gui analysis7/31/2023 Thus, a large proportion of resources in crop improvement programs are invested toward better seeds. Most of the plant-based food that we eat is either seed or seed-derived products. In summary, we present a publicly available application that can be used to determine key yield-related traits in crops. We identified known loci for regulating seed length ( GS3) and width ( qSW5/GW5) in rice, which demonstrates the accuracy of this application to extract seed phenotypes and accelerate trait discovery. The derived seed-size traits, such as seed length, width, were used for genome-wide association analysis. We further validated the utility of this application by analyzing mature-rice seeds from 231 accessions in Rice Diversity Panel 1. The adaptability of SeedExtractor was demonstrated by analyzing scanned seeds from multiple crops. As this software is open-source, it can be modified by users to serve more specific needs. In this context, our application takes ∼2 s for analyzing an image, i.e., significantly less compared to the other tools. To address this need, we have developed an open-source graphical user interface (GUI) software, SeedExtractor that determines seed size and shape (including area, perimeter, length, width, circularity, and centroid), and seed color with capability to process a large number of images in a time-efficient manner. 3Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, United StatesĪccurate measurement of seed size parameters is essential for both breeding efforts aimed at enhancing yields and basic research focused on discovering genetic components that regulate seed size.2Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE, United States.1Department of Computer Science and Engineering, University of Nebraska-Lincoln, Lincoln, NE, United States. Dhatt 2, Jaspreet Sandhu 2, Larissa Irvin 2, Gota Morota 3, Hongfeng Yu 1 and Harkamal Walia 2* Feiyu Zhu 1†, Puneet Paul 2†, Waseem Hussain 2‡, Kyle Wallman 2, Balpreet K.
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